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In assembling this book, Protocols for Nucleic Acid Analysis by Nonradioactive Probes, I have endeavored to select protocols that have wide applicability. My aim in doing so is to allow nucleic acid analysis to move from the confines of specialized research labora- ries and into general purpose and teaching laboratories. In particular, disciplines such as population biology and clinical diagnostics (where the application of the technology is important, not the technology itself) will find the newer techniques easier to perform with the qu- tity of samples that are normally required in these fields. Die-hard molecular biologists with hot fingers will find, by trying some of the protocols in this book, that nonradioactive protocols are normally faster than their radioactive brethren, and give more reliable results. An expanded description of the subject areas covered by this book is given in Chapter 1. The technology herein has very broad application; for instance, in conventional molecular biology research, population biology, plant and animal breeding, genetic mapping, paternity testing, forensics, prenatal diagnosis, and clinical and food microbiology. I have tried to include comprehensive protocols for both basic and more complex analyses. There are chapters dealing with the fundamentals of nucleic acid extraction from plants and a- mals, and with the procedures necessary to immobilize nucleic acids on solid supports. A range of labeling procedures is included, f- lowed by a selection of hybridization procedures.
Molecular biology --- Nucleic acid probes --- Nucleic acids --- Oligosondes --- Acides nucléiques --- Analysis --- Analyse --- Nucleic Acids --- Nucleic Acid Probes --- Nucleic Acids, Nucleotides, and Nucleosides --- Molecular Probes --- Chemicals and Drugs --- Laboratory Chemicals --- Specialty Uses of Chemicals --- Chemical Actions and Uses --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Nucleic acid probes. --- Analysis. --- Acides nucléiques --- Life sciences. --- Cell biology. --- Life Sciences. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biosciences --- Sciences, Life --- Science --- Cytology.
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Mark Chase There are many literature resources available to molecular biologists wishing to assess genetic variation, but the myriad of techniques and approaches potentially available to the plant breeder and the evolutionary biologist is truly bewildering, and most have never been evaluated side-by-side on the same sets of samples. Additionally, it is often not recognized that tools that are useful for breeders can often be adapted for use in evolutionary studies and vice versa, but this is generally the case. The borderline between population genetics and phylogenetics is vague and difficult to assess, and a combination of both types of tools is best when it is not clear with which area one is dealing. Furthermore, it is not now appropriate to use just one type of marker in any kind of study; most markers have the potential to misinform under certain conditions, so it is always wise to incorporate at least two different types of assessments into any project. This volume is designed to facilitate this sort of multiple approach and provides comparative data on most currently available methods so that researchers can more intelligently select those appropriate to their area of interest, regardless of whether it is in the realm of breeding or evolutionary biology.
Molecular probes. --- Sondes moléculaires --- Analyse biologique --- Biological analysis --- Arn ribosomal --- ribosomal RNA --- Extraction --- Polymorphisme --- Polymorphism --- Stabilité génétique --- Genetic stability --- 575.858 --- Species. Speciation --- 575.858 Species. Speciation --- Sondes moléculaires --- plants --- animals --- taxonomy --- Biodiversity --- ADN --- DNA --- PCR --- Chromatography --- Indicator organisms --- case studies --- 574.9 --- 574.472 --- 577.2 --- 577.2 Molecular bases of life. Molecular biology --- Molecular bases of life. Molecular biology --- 574.472 Biodiversity --- 574.9 Biogeography in general. Geographical distribution of organisms --- Biogeography in general. Geographical distribution of organisms --- Molecular biology --- Biologie moléculaire --- Measurement. --- Technique. --- Technique --- DNA. --- Nature conservation. --- Animal anatomy. --- Evolutionary biology. --- Nature Conservation. --- Animal Anatomy / Morphology / Histology. --- Evolutionary Biology. --- Animal evolution --- Animals --- Biological evolution --- Darwinism --- Evolutionary biology --- Evolutionary science --- Origin of species --- Biology --- Evolution --- Biological fitness --- Homoplasy --- Natural selection --- Phylogeny --- Animal anatomy --- Physiology --- Conservation of nature --- Nature --- Nature protection --- Protection of nature --- Conservation of natural resources --- Applied ecology --- Conservation biology --- Endangered ecosystems --- Natural areas --- Anatomy --- Conservation --- Biological diversification --- Biological diversity --- Biotic diversity --- Diversification, Biological --- Diversity, Biological --- Biocomplexity --- Ecological heterogeneity --- Numbers of species
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